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Biotechnol Bioeng. 2003 Aug 20;83(4):395-9.

Critical evaluation of sampling techniques for residual glucose determination in carbon-limited chemostat culture of Saccharomyces cerevisiae.

Author information

1
Kluyver Laboratory for Biotechnology, 67 Julianalaan, 2628BC Delft, The Netherlands. M.Mashego@TNW.TUDelft.nl

Abstract

In this paper, three sampling techniques for rapid quenching of cellular metabolism and subsequent separation of cells from fermentation broth are compared: (i) quick freezing of fermentation broth directly in liquid nitrogen; (ii) quenching metabolism by exposing the fermentation broth to stainless steel beads (4-mm diameter) in a filter syringe precooled to -18 degrees C; and (iii) withdrawal of the filtrate through a 0.45 microm filter attached to a syringe and a needle inserted directly into the fermentor. It was concluded that use of liquid nitrogen as a quenching method to rapidly arrest cellular metabolism, for quantitative analysis of extracellular glucose, is not a very reliable method and that the filter syringe steel beads work very well.

PMID:
12800134
DOI:
10.1002/bit.10683
[Indexed for MEDLINE]

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