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FEMS Microbiol Lett. 2003 Jun 6;223(1):15-20.

Isolation of high molecular weight DNA from soil for cloning into BAC vectors.

Author information

1
Department of Biological Sciences, University of Warwick, Gibbet Hill Rd, Coventry CV4 7AL, UK. aberry@bio.warwick.ac.uk

Abstract

Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified.

PMID:
12798994
DOI:
10.1016/S0378-1097(03)00248-9
[Indexed for MEDLINE]
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