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Plant Cell Rep. 2003 Aug;21(12):1143-52. Epub 2003 May 28.

Use of an inducible reporter gene system for the analysis of auxin distribution in the moss Physcomitrella patens.

Author information

1
Plant Biotechnology, Freiburg University, Schaenzlestr. 1, 79104, Freiburg, Germany.

Abstract

The plant hormone auxin plays a major role in a variety of growth and developmental responses, even in the more ancient plants-for example, cell differentiation in mosses. Nevertheless, almost nothing is known about the distribution of auxin during moss development. To address this question, we characterised auxin distribution in the moss Physcomitrella patens using auxin-inducible reporter gene systems. Stable transgenic Physcomitrella plants were produced expressing the beta-glucuronidase (GUS) gene driven by the auxin-inducible promoters GH3 and DR5, respectively. Both fusions showed remarkable differences with respect to auxin-induced promoter strength and expression kinetics. A detailed characterisation of the GUS expression pattern in different developmental stages revealed that the highest auxin concentrations were in dividing and ontogenetic young cells.

PMID:
12789498
DOI:
10.1007/s00299-003-0646-1
[Indexed for MEDLINE]

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