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J Muscle Res Cell Motil. 2002;23(5-6):397-407.

Optical tweezers stretching of chromatin.

Author information

1
Department of Applied Physics and MESA Research Institute, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands. l.h.pope@tn.utwente.nl

Abstract

Recently significant success has emerged from exciting research involving chromatin stretching using optical tweezers. These experiments, in which a single chromatin fibre is attached by one end to a micron-sized bead held in an optical trap and to a solid surface or second bead via the other end, allows manipulation and force detection at a single-molecule level. Through force-induced stretching of chromatin, mechanical properties, specific intermolecular bond strengths and DNA-protein association and dissociation kinetics have been determined. These studies will be extremely fruitful in terms of understanding the function of chromatin structure and its dynamics within the cell.

PMID:
12785093
[Indexed for MEDLINE]

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