Abstract
We examined the possibility of measuring the organophosphorus aromatic nitro insecticides metaphos and sumithion as well as their hydrolysis product p-nitrophenol (PNP) by the specific respiratory activity (SRA) of Pseudomonas putida C-11, P. putida BA-11, and Acinetobacter calcoaceticum A-122. The plots of cellular SRA against the two insecticides and PNP were linear over the ranges of 0.5-2.5 microM for P. putida C-11 and BA-11 and 0.5-1.0 microM for A. calcoaceticum A-122. P. putida BA-11 showed the greatest respiratory-response selectivity in the determination of the test substrates. We made comparison studies of the SRA of cells immobilised by two methods: carrier-surface adsorption and inclusion in various gels. We discuss the feasibility of developing a microbial sensor system for the determination of metaphos, sumithion, and PNP in aqueous media.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Acinetobacter calcoaceticus / chemistry
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Acinetobacter calcoaceticus / physiology*
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods*
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Cells, Immobilized
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Electrochemistry / instrumentation
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Electrochemistry / methods
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Fenitrothion / analysis*
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Fenitrothion / metabolism
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Hydrocarbons, Aromatic / analysis
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Hydrocarbons, Aromatic / metabolism
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Insecticides / analysis
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Insecticides / metabolism
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Methyl Parathion / analysis*
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Methyl Parathion / metabolism
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Nitrogen Compounds / analysis
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Nitrogen Compounds / metabolism
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Nitrophenols / analysis*
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Nitrophenols / metabolism
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Oxygen / analysis*
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Oxygen / metabolism
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Pseudomonas putida / chemistry
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Pseudomonas putida / physiology*
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Hydrocarbons, Aromatic
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Insecticides
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Nitrogen Compounds
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Nitrophenols
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Methyl Parathion
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Oxygen
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Fenitrothion
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4-nitrophenol