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J Microbiol Methods. 2003 Aug;54(2):269-76.

A microtiter-plate screening method for biofilm disinfection and removal.

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Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717, USA.


A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents. Biofilms of Pseudomonas aeruginosa or Staphylococcus epidermidis were grown in 96-well plates, treated with an agent, then stained with either the biomass indicator crystal violet or the respiratory indicator 5-cyano-2,3-ditolyl tetrazolium chloride. This rapid screening method is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments. Using these assays, agents can be ranked by their ability to remove or kill biofilm.

[Indexed for MEDLINE]

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