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Int J Food Microbiol. 2003 Jul 25;84(2):189-96.

Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences.

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Department of Biology, University of Oulu, P.O. Box 3000, Finland.


PCR primers derived from Lactobacillus rhamnosus phage Lc-Nu genome were used to screen the presence of phage-related sequences in Lb. rhamnosus strains. Several primer pairs derived from structural and replication gene regions of phage Lc-Nu amplified PCR products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 Lb. rhamnosus strains. Strain-specific PCR primers for three probiotic Lb. rhamnosus strains were derived from these phage-related sequences for identification and detection purposes. Specificity of these primers was tested against 11 Lb. rhamnosus strains and over 40 other bacterial strains.

[Indexed for MEDLINE]

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