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Mol Biotechnol. 2003 Jul;24(3):303-8.

Automated pneumococcal MLST using liquid-handling robotics and a capillary DNA sequencer.

Author information

1
Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow, G21 3UW, UK.

Abstract

Multilocus sequence typing (MLST) is used by the Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) as a routine method for the characterization of certain bacterial pathogens. The SMPRL recently started performing MLST on strains of Streptococcus pneumoniae, and here we describe a fully automated method for MLST using a 96-well-format liquid-handling robot and a 96-capillary automated DNA sequencer.

PMID:
12777696
DOI:
10.1385/MB:24:3:303
[Indexed for MEDLINE]

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