Format

Send to

Choose Destination
Science. 2003 May 30;300(5624):1434-6.

Water-soluble quantum dots for multiphoton fluorescence imaging in vivo.

Author information

1
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Abstract

The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such as living tissue. We characterized water-soluble cadmium selenide-zinc sulfide quantum dots for multiphoton imaging in live animals. These fluorescent probes have two-photon action cross sections as high as 47,000 Goeppert-Mayer units, by far the largest of any label used in multiphoton microscopy. We visualized quantum dots dynamically through the skin of living mice, in capillaries hundreds of micrometers deep. We found no evidence of blinking (fluorescence intermittency) in solution on nanosecond to millisecond time scales.

PMID:
12775841
DOI:
10.1126/science.1083780
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center