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Mol Cell. 2003 May;11(5):1301-9.

Independent recruitment in vivo by Gal4 of two complexes required for transcription.

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Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10021, USA.


We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.

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