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BMC Mol Biol. 2003 May 26;4:6. Epub 2003 May 26.

The kinase MSK1 is required for induction of c-fos by lysophosphatidic acid in mouse embryonic stem cells.

Author information

1
Interfakultäres Institut für Zellbiologie, Abteilung Molekularbiologie, Universität Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany. schuck@mpi-cbg.de

Abstract

BACKGROUND:

The regulation of the immediate-early gene c-fos serves as a paradigm for signal-activated gene induction. Lysophosphatidic acid is a potent serum-borne mitogen able to induce c-fos.

RESULTS:

Analysing the signalling events following stimulation of mouse embryonic stem cells with serum and lysophosphatidic acid, we show that the extracellular signal-regulated kinase (ERK) pathway is involved in mediating c-fos induction. We demonstrate that the ERK-activated kinase MSK1 is required for full c-fos promoter activation, as well as for the phosphorylation of cAMP-responsive element (CRE) binding proteins. We propose that MSK1 contributes to ERK-mediated c-fos promoter activation by targeting CRE binding proteins.

CONCLUSION:

These results show that MSK1 is an important ERK-activated mediator of mitogen-stimulated c-fos induction. In addition, they indicate that MSK1 could act through CRE binding proteins to achieve c-fos promoter activation. Thus, they further our understanding of the complex regulation of the model immediate-early gene c-fos.

PMID:
12769834
PMCID:
PMC161794
DOI:
10.1186/1471-2199-4-6
[Indexed for MEDLINE]
Free PMC Article
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