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Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):7081-6. Epub 2003 May 23.

Uniform polarity microtubule assemblies imaged in native brain tissue by second-harmonic generation microscopy.

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1
School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.

Abstract

Microtubule (MT) ensemble polarity is a diagnostic determinant of the structure and function of neuronal processes. Here, polarized MT structures are selectively imaged with second-harmonic generation (SHG) microscopy in native brain tissue. This SHG is found to colocalize with axons in both brain slices and cultured neurons. Because SHG arises only from noninversion symmetric structures, the uniform polarity of axonal MTs leads to the observed signal, whereas the mixed polarity in dendrites leads to destructive interference. SHG imaging provides a tool to investigate the kinetics and function of MT ensemble polarity in dynamic native brain tissue structures and other subcellular motility structures based on polarized MTs.

PMID:
12766225
PMCID:
PMC165833
DOI:
10.1073/pnas.0731953100
[Indexed for MEDLINE]
Free PMC Article
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