Format

Send to

Choose Destination
Trends Biochem Sci. 2003 May;28(5):244-9.

Standardization of protocols in cDNA microarray analysis.

Author information

1
European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. benes@embl-heidelberg.de

Abstract

Systematic variations can occur at various steps of a cDNA microarray experiment and affect the measurement of gene expression levels. Accepted standards integrated into every cDNA microarray analysis can assess these variabilities and aid the interpretation of cDNA microarray experiments from different sources. A universally applicable approach to evaluate parameters such as input and output ratios, signal linearity, hybridization specificity and consistency across an array, as well as normalization strategies, is the utilization of exogenous control genes as spike-in and negative controls. We suggest that the use of such control sets, together with a sufficient number of experimental repeats, in-depth statistical analysis and thorough data validation should be made mandatory for the publication of cDNA microarray data.

PMID:
12765836
DOI:
10.1016/S0968-0004(03)00068-9
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center