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FEMS Microbiol Lett. 2003 May 16;222(1):1-8.

The phosphotriesterase gene opdA in Agrobacterium radiobacter P230 is transposable.

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1
CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia. irene.horne@

Abstract

We report a transposase gene (tnpA) upstream of the opdA phosphotriesterase gene of Agrobacterium radiobacter P230, as well as inverted repeats indicative of insertion sequences, flanking the two genes. Both the tnpA gene and the inverted repeats resemble the Tn610 transposon from Mycobacterium fortuitum. Two additional putative open reading frames separate opdA and tnpA with inferred translation products with similarity to two proteins encoded on the Geobacillus stearothermophilus IS5376 transposon. To test the proposition that these genes were contained on a transposon, an artificial composite transposon was constructed. This artificial transposon was then delivered into Escherichia coli DH10beta cells. Transposition was demonstrated by the presence of opdA on the E. coli chromosome and confirmation of insertion by inverse polymerase chain reaction. The data presented suggest a possible role of transposition in the distribution of the opd/opdA genes across a wide range of soil bacteria.

PMID:
12757939
DOI:
10.1016/S0378-1097(03)00211-8
[Indexed for MEDLINE]
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