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Immunity. 2003 May;18(5):699-711.

Notch1 but not Notch2 is essential for generating hematopoietic stem cells from endothelial cells.

Author information

1
Department of Hematology and Oncology, Graduate School of Medicine, University of Tokyo, Japan.

Abstract

Hematopoietic stem cells (HSCs) are thought to arise in the aorta-gonad-mesonephros (AGM) region of embryo proper, although HSC activity can be detected in yolk sac (YS) and paraaortic splanchnopleura (P-Sp) when transplanted in newborn mice. We examined the role of Notch signaling in embryonic hematopoiesis. The activity of colony-forming cells in the YS from Notch1(-/-) embryos was comparable to that of wild-type embryos. However, in vitro and in vivo definitive hematopoietic activities from YS and P-Sp were severely impaired in Notch1(-/-) embryos. The population representing hemogenic endothelial cells, however, did not decrease. In contrast, Notch2(-/-) embryos showed no hematopoietic deficiency. These data indicate that Notch1, but not Notch2, is essential for generating hematopoietic stem cells from endothelial cells.

PMID:
12753746
DOI:
10.1016/s1074-7613(03)00117-1
[Indexed for MEDLINE]
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