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Plant Physiol. 2003 May;132(1):27-35.

Laser capture microdissection of cells from plant tissues.

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1
Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208104, New Haven, Connecticut 06520-8104, USA.

Abstract

Laser capture microdissection (LCM) is a technique by which individual cells can be harvested from tissue sections while they are viewed under the microscope, by tacking selected cells to an adhesive film with a laser beam. Harvested cells can provide DNA, RNA, and protein for the profiling of genomic characteristics, gene expression, and protein spectra from individual cell types. We have optimized LCM for a variety of plant tissues and species, permitting the harvesting of cells from paraffin sections that maintain histological detail. We show that RNA can be extracted from LCM-harvested plant cells in amount and quality that are sufficient for the comparison of RNAs among individual cell types. The linear amplification of LCM-captured RNA should permit the expression profiling of plant cell types.

PMID:
12746508
PMCID:
PMC1540312
DOI:
10.1104/pp.102.018127
[Indexed for MEDLINE]
Free PMC Article
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