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BJOG. 2003 May;110(5):467-74.

Dexamethasone and ursodeoxycholic acid protect against the arrhythmogenic effect of taurocholate in an in vitro study of rat cardiomyocytes.

Author information

1
Division of Medicine, Imperial College London, MRC Clinical Sciences Centre, UK.

Abstract

OBJECTIVE:

To establish whether the therapeutic agents ursodeoxycholic acid and dexamethasone protect cardiomyocytes from taurocholate-induced arrhythmias in an in vitro model.

DESIGN:

Laboratory study.

SETTING:

Imperial College London, Hammersmith Campus.

SAMPLE:

Neonatal rat cardiomyocytes.

METHODS:

Using scanning ion conductance microscopy, we measured the rate, rhythm, amplitude of contraction and calcium dynamics of ventricular myocytes from one to two day old rats. Cells were pre-incubated for 16 hours in dexamethasone (80 or 800 nM) or 0.1 mM ursodeoxycholic acid before adding taurocholate at different concentrations (0.3-4.5 mM).

MAIN OUTCOME MEASURES:

Changes in rate and amplitude of contraction, calcium dynamics and rhythm.

RESULTS:

Taurocholate at concentrations of up to 3 mM induces abnormal changes including reductions in rate, amplitude of contraction, abnormal calcium dynamics and dysrhythmias. Although dexamethasone had no immediate protective effect on these changes, pre-incubation with dexamethasone was protective. Ursodeoxycholic acid pre-incubation was protective at taurocholate concentrations up to 1 mM.

CONCLUSION:

The therapeutic agents dexamethasone and ursodeoxycholic acid appear protective against the arrhythmogenic effect of taurocholate on cardiomyocytes.

PMID:
12742331
[Indexed for MEDLINE]
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