Format

Send to

Choose Destination
Nat Immunol. 2003 Jun;4(6):565-72. Epub 2003 May 11.

NKG2D triggers cytotoxicity in mouse NK cells lacking DAP12 or Syk family kinases.

Author information

1
Department of Immunology, Pasteur Institute, 25-28 rue Dr Roux, 75015 Paris, France.

Abstract

In activated mouse natural killer (NK) cells, the NKG2D receptor associates with two intracellular adaptors, DAP10 and DAP12, which trigger phosphatidyl inositol 3 kinase (PI3K) and Syk family protein tyrosine kinases, respectively. Here we show that cytotoxicity, but not cytokine production, is triggered by NKG2D in activated NK cells lacking either DAP12 or the Syk family members Syk and ZAP70. Inhibition of PI3K blocks this cytotoxicity, suggesting that the DAP10-PI3K pathway is sufficient to initiate NKG2D-mediated killing of target cells. Our results highlight signaling divergence in the effector functions of NKG2D and indicate that alternative associations between a receptor and its adaptors may provide a single receptor with a dual 'on-switch', giving mouse NK cells more choices through which to trigger cytotoxicity.

Comment in

PMID:
12740576
DOI:
10.1038/ni930
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for eScholarship, California Digital Library, University of California
Loading ...
Support Center