Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common red cell enzymopathy among humans. G6PD deficiency was reported in India more than 30 years ago and about 13 biochemically characterized variants have been reported. We now describe the molecular characterization of a distinct biochemical variant from India which was previously characterized by us as a unique Class IV variant with fast electrophoretic mobility. The sequence of the G6PD gene from this variant has revealed the presence of a novel 989 G --> A mutation in exon 9 with a predicted amino acid change of Arg330His.