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Biol Proced Online. 1999 Oct 4;2:1-38.

Yeast Two-Hybrid: State of the Art.

Author information

1
DEVGEN NV. Technologiepark 9, 9052 Zwijnaarde-Gent. Belgium.; Department of Molecular Biology. Flanders Interuniversity Institute for Biotechnology and University of Ghent, B-9000, Ghent. Belgium. wim.vancriekinge@devgen.com

Abstract

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination.

PMID:
12734586
PMCID:
PMC140126
DOI:
10.1251/bpo16

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