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FEMS Microbiol Lett. 2003 Apr 25;221(2):249-55.

Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation.

Author information

1
Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche Vigne et Vins d'Alsace, Colmar, France. legras@colmar.inra.fr

Abstract

A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis using the newly designed primers.

PMID:
12725935
DOI:
10.1016/S0378-1097(03)00205-2
[Indexed for MEDLINE]
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