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Methods. 2003 Mar;29(3):270-81.

Mapping T cell epitopes by flow cytometry.

Author information

1
Department of Medical Immunology, Charité, Humboldt Universität zu Berlin, Schumannstrasse 21, 10117 Berlin, Germany.

Abstract

Epitope mapping by flow cytometry is a very modern approach that not only identifies T-cell epitopes but simultaneously allows for detailed analysis of the responding T-cell subsets including lineage, activation marker expression, and other markers of interest. The most frequently used approach is based on the identification of intracellular cytokines in secretion-inhibited activated T cells following stimulation with peptides or peptide pools. A more recently developed assay analyzes T-cell proliferation by measuring the decrease in carboxyfluorescein diacetate succinimidyl ester staining in proliferated cells. This article includes information on peptide configuration, a section on the design and efficient application of peptide pools, and working laboratory protocols for both assays.

PMID:
12725792
[Indexed for MEDLINE]

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