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Biochem Biophys Res Commun. 2003 Apr 25;304(1):11-7.

Molecular cloning of a cDNA encoding a soluble protein in the coral exoskeleton.

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Division of Marine Biosciences, Ocean Research Institute, The University of Tokyo, 1-15-1 Minamidai, Nakano, 168-8639, Tokyo, Japan.


Organic substances were extracted from the calcified exoskeleton of the reef coral Galaxea fascicularis. In an SDS-PAGE analysis of the extract, a protein with an apparent molecular mass of 53 kDa was detected as well as two other weaker bands. A Ca2+ overlay analysis failed to find a Ca2+-binding protein in the extract. Periodic acid Schiff staining indicated that the 53 kDa protein was glycosylated. A cDNA containing the entire open reading frame for this protein was obtained. Analysis of the deduced protein sequence suggests that the protein, named galaxin, is synthesized as a precursor consisting of a signal peptide, a propeptide sequence, and a mature protein of 298 amino acids. Galaxin exhibits a novel amino acid sequence which is characterized by a tandem repeat structure. Galaxin transcripts were detected in the adult coral, but not in planktonic larvae.

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