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J Cell Biol. 2003 Apr 14;161(1):27-32.

RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis.

Author information

1
Cell and Developmental Biology Biology, University of California, San Diego, La Jolla, CA 92093-0347, USA.

Abstract

Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not contain activated MEK1. MEK1-dependent Golgi complex fragmentation is through activation by RAF1 and not MEK1 kinase 1. We propose that a RAF1-dependent activation of MEK1 and its presence on the Golgi apparatus in late prophase is required for Golgi complex fragmentation.

PMID:
12695496
PMCID:
PMC2172875
DOI:
10.1083/jcb.200208099
[Indexed for MEDLINE]
Free PMC Article

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