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Methods. 2003 May;30(1):42-8.

Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins.

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1
Laboratory for Neuronal Circuit Dynamics, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan. knopfel@brain.riken.go.jp

Abstract

Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique.

PMID:
12695102
[Indexed for MEDLINE]
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