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Plant J. 2003 Apr;34(2):249-55.

High-Cot sequence analysis of the maize genome.

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1
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.

Erratum in

  • Plant J. 2003 Nov;36(3):430.

Abstract

Higher eukaryotic genomes, including those from plants, contain large amounts of repetitive DNA that complicate genome analysis. We have developed a technique based on DNA renaturation which normalizes repetitive DNA, and thereby allows a more efficient outcome for full genome shotgun sequencing. The data indicate that sequencing the unrenatured outcome of a Cot experiment, otherwise known as High-Cot DNA, enriches genic sequences by more than fourfold in maize, from 5% for a random library to more than 20% for a High-Cot library. Using this approach, we predict that gene discovery would be greater than 95% and that the number of sequencing runs required to sequence the full gene space in maize would be at least fourfold lower than that required for full-genome shotgun sequencing.

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