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Fungal Genet Biol. 2003 Apr;38(3):327-32.

Circadian and light-induced expression of luciferase in Neurospora crassa.

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Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA.


We have constructed a plasmid vector for expressing firefly luciferase in Neurospora crassa under control of the light- and clock-regulated ccg-2 (eas) promoter. The sequence of the luciferase gene in the vector has been modified to reflect the N. crassa codon bias. Both light-induced activity and circadian activity are demonstrated. Expression of luciferase in strains carrying mutant frequency alleles shows appropriate period length alterations. These data demonstrate that luciferase is a sensitive reporter of gene expression in N. crassa. Our results also show that the modified luciferase is expressed in Aspergillus nidulans.

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