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Infect Control Hosp Epidemiol. 2003 Mar;24(3):191-4.

Can Whipple's disease be transmitted by gastroscopes?

Author information

1
Unité des Rickettsies, CNRS UMR 6020, Faculté de médecine, 27 Boulevard Jean Moulin, 13385 Marseille 05, France.

Abstract

OBJECTIVE:

To determine whether disinfection protocols currently used for gastroscopes are effective against cultures of Tropheryma whipplei.

DESIGN:

The bactericidal activity of 2% glutaraldehyde and two peracetic acids on the Twist-Marseille strain of T. whipplei grown in cell monolayers was determined.

PATIENTS:

Two patients who were diagnosed as having Whipple's disease 3 years after they had had intestinal biopsies.

RESULTS:

The disinfectants reduced bacteria by approximately 2 log to 3 log10 after 5 to 60 minutes of contact.

CONCLUSION:

The bactericidal activity of a disinfectant is usually considered significant if it causes a 5 log10 or greater reduction in viable bacterial titers. Disinfecting gastroscopes with 2% glutaraldehyde or peracetic acids for 20 minutes may be insufficient to prevent transmission of T. whipplei on the instruments or stop false-positive results on polymerase chain reaction.

Comment in

PMID:
12683510
DOI:
10.1086/502188
[Indexed for MEDLINE]

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