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FEBS Lett. 2003 Apr 10;540(1-3):245-50.

Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells.

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1
Department of Bioscience, University of Strathclyde, 204 George Street, Glasgow G1 1XW, UK.

Abstract

Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (HL60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10-100 microM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

PMID:
12681516
[Indexed for MEDLINE]
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