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FEBS Lett. 2003 Apr 10;540(1-3):35-40.

Constitutive overexpression of barley 4-hydroxyphenylpyruvate dioxygenase in tobacco results in elevation of the vitamin E content in seeds but not in leaves.

Author information

1
Institute of Botany, University of Kiel, Olshausenstrasse 40, D-24098 Kiel, Germany.

Abstract

With the aim to enhance the plant vitamin E content, the barley gene encoding 4-hydroxyphenylpyruvate dioxygenase was overexpressed in tobacco plants under control of the 35S promoter. Transgenic lines have a higher capacity for homogentisate biosynthesis as evident by a more than 10-fold higher resistance towards the bleaching herbicide sulcotrione. Seeds from transgenic lines have an up to two-fold enhanced level of vitamin E without a change in the ratio of gamma-tocopherol and gamma-tocotrienol. While the vitamin E content is not affected in leaves, the level of plastoquinone is enhanced in leaves of transgenic lines during leaf senescence.

PMID:
12681479
DOI:
10.1016/s0014-5793(03)00166-2
[Indexed for MEDLINE]
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