Format

Send to

Choose Destination
See comment in PubMed Commons below
FEBS Lett. 2003 Apr 10;540(1-3):3-6.

Oxidative stress in cell culture: an under-appreciated problem?

Author information

1
Department of Biochemistry, Faculty of Medicine, MD 7 #03-07, 8 Medical Drive, Singapore 117597, Singapore. bchbh@nus.edu.sg

Abstract

Cell culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, cells in culture may behave differently from cells in vivo in many ways. One of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that survive and grow in culture might use ROS-dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that cell culture media can catalyse the oxidation of compounds added to them, resulting in apparent cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on cells in culture.

PMID:
12681474
[Indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center