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Exp Cell Res. 2003 Apr 15;285(1):99-106.

LIM-only protein FHL3 interacts with CDC25B2 phosphatase.

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Laboratoire de Biologie Cellulaire et Moléculaire du Contrôle de la Prolifération, CNRS UMR5088, Université Paul Sabatier, IFR 109 "Exploration Fonctionnelle des Génomes," 118 Route de Narbonne, Batiment 4R3b1-31062, Toulouse Cedex, France.


LIM domain proteins are important regulators of the growth, determination, and differentiation of cells. In this report, FHL3 (human four-and-a-half LIM-only protein 3) is shown to interact with human phosphatase CDC25B, a cell cycle regulator involved in the control of G2/M. We found that this interaction was specific to the CDC25B2 isoform. Deletion and point mutation studies indicated that the second LIM domain of FHL3 was essential for this interaction. FRET experiments in C2C12 cells showed that, although both proteins were colocated in the cytoplasm and the nucleus, they interacted only in the nucleus. Finally, we showed that FHL3 binding impaired neither CDC25B2 phosphatase activity nor its localization. Further work is now needed to elucidate the consequences of this interaction on myoblast fate decision and cycle control.

[Indexed for MEDLINE]

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