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Rapid Commun Mass Spectrom. 2003;17(8):845-50.

Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry.

Author information

1
Centre for Science and Technology in Medicine, School of Postgraduate Medicine, Keele University, Thornburrow Drive, Hartshill, Stoke-on-Trent ST4 7QB, UK. d.smith@bemp.keele.ac.uk

Abstract

The production of volatile compounds from cancer cell lines in vitro has been investigated using selected ion flow tube mass spectrometry (SIFT-MS). This technique enables on-line quantitative analyses of the headspace above cell/medium cultures. This paper reports the discovery that acetaldehyde is released by the lung cancer cell lines SK-MES and CALU-1. The concentration of acetaldehyde in the headspace of the medium/cell culture was measured after 16 h incubation at 37 degrees C and found to be proportional to the number of cancer cells in the medium (typically 10(8)). From these data, the acetaldehyde production rates of the SK-MES cells and the CALU-1 cells in vitro are determined to be 1 x 10(6) and 1.5-3 x 10(6) molecules/cell/min, respectively. The potential value of this new technique in cell biology and in industrial cell biotechnology is discussed.

PMID:
12672140
DOI:
10.1002/rcm.984
[Indexed for MEDLINE]

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