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Sex Transm Dis. 2003 Apr;30(4):310-4.

Time course of seroconversion by HerpeSelect ELISA after acquisition of genital herpes simplex virus type 1 (HSV-1) or HSV-2.

Author information

1
Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA. rashle@chmc.org

Abstract

BACKGROUND:

HerpeSelect HSV-1 and HSV-2 ELISAs are glycoprotein G-based, type-specific antibody detection tests that are approved by the US Food and Drug Administration for diagnosis of genital herpes.

GOAL:

The goal was to determine seroconversion times by means of HerpeSelect ELISAs.

STUDY DESIGN:

Four-hundred thirteen sera from 113 patients with recently acquired genital herpes were tested by HerpeSelect ELISAs and Western blot (WB). Thirty-one patients had primary genital HSV-1 (group 1), 56 had primary HSV-2 (group 2), and 26 had prior HSV-1 antibodies and newly acquired HSV-2 (group 3).

RESULTS:

Median interval from onset of symptoms to seroconversion was 25 days, as determined by HerpeSelect HSV-1, versus 33 days by WB for group 1; 21 days by HerpeSelect HSV-2 versus 40 days by WB (group 2; P = 0.0005); and 23 days by HerpeSelect HSV-2 ELISA versus 47 days by WB (group 3; P = 0.02). In long-term follow-up, transient reversion to HerpeSelect negativity occurred in 3 of 31 HSV-1-infected subjects (10%) and in 2 of 82 HSV-2-infected subjects (2%).

CONCLUSION:

Seroconversion to HSV-2 was determined faster by HerpeSelect than by WB.

PMID:
12671550
[Indexed for MEDLINE]

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