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Biophys J. 2003 Apr;84(4):2467-73.

Increased bending rigidity of single DNA molecules by H-NS, a temperature and osmolarity sensor.

Author information

1
Department of Physics of Complex Systems, The Weizmann Institute of Science, Rehovot 76100, Israel.

Abstract

Histonelike nucleoid structuring protein (H-NS) is an abundant prokaryotic protein participating in nucleoid structure, gene regulation, and silencing. It plays a key role in cell response to changes in temperature and osmolarity. Force-extension measurements of single, twist-relaxed lambda-DNA-H-NS complexes show that these adopt more extended configurations compared to the naked DNA substrates. Crosslinking indicates that H-NS can decorate DNA molecules at one H-NS dimer per 15-20 bp. These results suggest that H-NS polymerizes along DNA, forming a complex of higher bending rigidity. These effects are not observed above 32 degrees C or at high osmolarity, supporting the hypothesis that a direct H-NS-DNA interaction plays a key role in gene silencing. Thus, we propose that H-NS plays a unique structural role, different from that of HU and IHF, and functions as one of the environmental sensors of the cell.

PMID:
12668454
PMCID:
PMC1302812
DOI:
10.1016/S0006-3495(03)75051-6
[Indexed for MEDLINE]
Free PMC Article

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