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J Immunol Methods. 2003 Apr 1;275(1-2):113-21.

A simple method for detecting up to five immunofluorescent parameters together with DNA staining for cell cycle or viability on a benchtop flow cytometer.

Author information

1
Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.

Abstract

In this manuscript, we describe modifications to a commercial three-laser benchtop flow cytometer, as well as relevant biological methods, that allow analysis of up to five immunofluorescent parameters together with an ultraviolet (UV)-excitable DNA stain. This method allows expanded capacity for multiparameter immunophenotyping of complex mixed cell populations, together with accurate measurements of DNA content (cell cycle) or cell viability, on a stable, end-user operated platform.

PMID:
12667675
DOI:
10.1016/s0022-1759(03)00009-7
[Indexed for MEDLINE]

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