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Ann Clin Biochem. 2003 Mar;40(Pt 2):191-3.

Stability of parathyroid hormone ex vivo in haemodialysis patients.

Author information

1
Department of Clinical Biochemistry, East Kent Hospitals NHS Trust, William Harvey Hospital, Ashford TN24 0LZ, UK. teresa.teal@ekht.nhs.uk

Abstract

BACKGROUND:

The stability of parathyroid hormone (PTH) in blood ex vivo is a significant practical problem for laboratories and clinicians. Several studies have suggested that PTH is more stable in blood collected into a potassium edetate (EDTA) preservative.

METHODS:

To confirm that this was applicable to renal dialysis patients using our assay (Nichols chemiluminescence), we examined PTH stability in 13 patients with end-stage renal failure using three different blood collection tubes.

RESULTS:

PTH remained stable in EDTA plasma for up to 48 h at room temperature. PTH was significantly reduced in serum collected into plain tubes after 2 h, and after 4 h in serum collected into serum separator tubes, at room temperature.

CONCLUSION:

In the assessment of renal osteodystrophy, the use of EDTA plasma can confer significant benefit, especially in busy laboratories where rapid frozen separation of blood may be hard to achieve.

PMID:
12662412
DOI:
10.1258/000456303763046175
[Indexed for MEDLINE]

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