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Gene. 2003 Mar 13;306:99-103.

Subgenome-specific markers in allopolyploid cotton Gossypium hirsutum: implications for evolutionary analysis of polyploids.

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Department of Biology, Texas Tech University, Lubbock, TX 79409, USA.


We developed a set of genetic markers specific to the A and D genome types of cotton using representational difference analysis (RDA). These markers produce amplification products with genomic DNA from allotetraploid cotton Gossypium hirsutum. One of the markers is a polymorphic amplified restriction fragment (PARF) - a sequence found in both A and D genomes but differently flanked by restriction sites. Results of phylogenetic analysis of the PARF sequences from diploid cottons and from allotetraploid G. hirsutum agree with a previous observation of the interlocus concerted evolution (sequences corresponding to A and D genomes are homogenized to a D genome-type sequence). Our study shows how RDA can be used to develop genome-specific markers that can be used to study molecular evolution of allopolyploids.

[Indexed for MEDLINE]

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