Format

Send to

Choose Destination
Opt Lett. 2003 Jan 15;28(2):69-71.

Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging.

Author information

1
Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.

Abstract

We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40 microm x 60 microm x 3 microm. With the technique presented, the structure-mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.

PMID:
12656488
DOI:
10.1364/ol.28.000069
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Optical Society of America
Loading ...
Support Center