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J Antimicrob Chemother. 2003 Apr;51(4):843-7. Epub 2003 Feb 25.

In vitro effect on Cryptosporidium parvum of short-term exposure to cathelicidin peptides.

Author information

1
Institute of Infectious Diseases and Public Health, University of Ancona, Italy. anconacmi@interfree.it

Abstract

Two laboratory methods, a cell culture system and double fluorogenic staining, were used to study the viability and infective ability of Cryptosporidium parvum sporozoites and oocysts after short-term exposure to four cathelicidin peptides. The compounds, SMAP-29, BMAP-28, PG-1 and Bac7(1-35), exerted a strong cytotoxic effect on sporozoites, but did not affect the viability and function of oocysts consistently. Overall, in the sporozoite series, a percentage of the viable population decreased rapidly to less than detectable levels after 15 and 60 min exposure to the peptides at concentrations of 100 and 10 micro g/mL, respectively. In the oocyst series, no compound produced complete inhibition of parasite growth: 60-85% of the oocyst population was viable after 180 min exposure at 100 micro g/mL. SMAP-29 exerted the highest activity against both sporozoites and oocysts.

PMID:
12654759
DOI:
10.1093/jac/dkg149
[Indexed for MEDLINE]

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