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Nat Neurosci. 2003 May;6(5):453-60.

Otx dose-dependent integrated control of antero-posterior and dorso-ventral patterning of midbrain.

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MRC Centre for Developmental Neurobiology, New Hunt's House, 4th Floor, King's College London, Guy's Campus, London Bridge, London SE1 1UL, UK.


Organizing centers emit signaling molecules that specify different neuronal cell types at precise positions along the anterior-posterior (A-P) and dorsal-ventral (D-V) axes of neural tube during development. Here we report that reduction in Otx proteins near the alar-basal plate boundary (ABB) of murine midbrain resulted in a dorsal shift of Shh expression, and reduction in Otx proteins at the midbrain-hindbrain boundary (MHB) resulted in an anterior expansion of the Fgf8 domain. Our data thus indicate that an Otx dose-dependent repressive effect coordinates proper positioning of Shh and Fgf8 expression. Furthermore, this control is effective for conferring proper cell identity in the floor-plate region of midbrain and does not require an Otx2-specific property. We propose that this mechanism may provide both A-P and D-V positional information to neuronal precursors located within the midbrain.

[Indexed for MEDLINE]

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