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FEBS Lett. 2003 Mar 27;539(1-3):68-72.

Cu,Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis.

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Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Apdo. Postal 510-3, Cuernavaca 62250, Morelos, Mexico.


Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn-superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N-acetylcysteine to the medium. Pretreatment with MnCl(2), a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A-cAMP and SKN7 pathways.

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