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J Chromatogr A. 2003 Feb 21;988(1):95-105.

Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography.

Author information

1
Mass Spectrometry Research Unit, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK. 183366@swansea.ac.uk

Abstract

Four flavonoids, chrysin, baicalein, baicalein-7-O-glucoside, baicalein-7-O-diglucoside (Oroxylin B) and one unknown flavonoid have been isolated and purified for the first time in the seeds of Oroxylum indicum by high-speed counter-current chromatography with a two-phase solvent system composed of chloroform-methanol-water (8:10:5, v/v). Then, 50 mg baicalein-7-O-glucoside, 10.5 mg baicalein-7-O-diglucoside, 4.5 mg chrysin-7-O-diglucoside, 25 mg baicalein and 45 mg chrysin could be obtained after injecting 20 mg/ml sample extract ten times and their purities were 96, 90, 85, 95 and 98%, respectively. All these constituents were identified by high-performance liquid chromatography-mass spectrometry and nuclear magnetic resonance.

PMID:
12647824
DOI:
10.1016/s0021-9673(02)01954-4
[Indexed for MEDLINE]

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