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Hereditas. 2002;137(2):145-8.

New C-band protocol by heat denaturation in the presence of formamide.

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1
Departamento de Psicobiologia, Facultad Ciencias de la Educación, Universidad de A Coruña, Coruña, Spain.

Abstract

C-banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C-banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5% Ba(OH)2 at 50 degrees C for 5-15 min and subsequent incubation in salt solution (2 x SSC at 60 degrees C for 1 h). We here present a new, easy and reliable technique for C-banding, which basically involves heat denaturation of chromosomal DNA in the presence of formamide and incubation in 2 x SSC at room temperature.

PMID:
12627841
[Indexed for MEDLINE]
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