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Glycobiology. 2003 Mar;13(3):169-77. Epub 2002 Oct 30.

Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins.

Author information

1
Unité Des Aspergillus, Institut Pasteur, 25 Rue Du Docteur Roux, 75724 Paris Cedex 15, France. tfontain@pasteur.fr

Abstract

Glycosylphosphatidylinositol (GPI)-anchored proteins have been identified in all eukaryotes. In fungi, structural and biosynthetic studies of GPIs have been restricted to the yeast Saccharomyces cerevisiae. In this article, four GPI-anchored proteins were purified from a membrane preparation of the human filamentous fungal pathogen Aspergillus fumigatus. Using new methodology applied to western blot protein bands, the GPI structures were characterized by ES-MS, fluorescence labeling, HPLC, and specific enzymatic digestions. The phosphatidylinositol moiety of the A. fumigatus GPI membrane anchors was shown to be an inositol-phosphoceramide containing mainly phytosphingosine and monohydroxylated C24:0 fatty acid. In constrast to yeast, only ceramide was found in the GPI anchor structures of A. fumigatus, even for Gel1p, a homolog of Gas1p in S. cerevisiae that contains diacylglycerol. The A. fumigatus GPI glycan moiety is mainly a linear pentomannose structure linked to a glucosamine residue: Manalpha1-3Manalpha1-2Manalpha1-2Manalpha1-6Manalpha1-4GlcN.

PMID:
12626404
DOI:
10.1093/glycob/cwg004
[Indexed for MEDLINE]

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