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Scand Cardiovasc J. 2002 Dec;36(6):368-72.

The kinase insert domain-containing receptor (KDR) is regulated by shear stress.

Author information

1
Department of Vascular Surgery, The Wallenberg Laboratory, Bruna Stråket 11B, Plan 1, Sahlgrenska University Hospital, SE-413 45 Gothenburg, Sweden.

Abstract

OBJECTIVE:

Intimal hyperplasia develops in areas with low shear stress. The aim of the present study was to investigate if the mRNA expression of vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase-1 receptor (Flt-1) and kinase insert domain-containing receptor (KDR) is regulated by shear stress.

DESIGN:

Endothelial cells from human umbilical veins were in an in vitro system subjected to different levels of shear stress during 1 and 12 h. The mRNA expression of VEGF, Flt-1 and KDR was measured with RT-PCR. eNOS served as positive control and actin as housekeeping gene.

RESULTS:

The KDR expression was isolated upregulated 3-4 times after 12 h exposure to high shear stress.

CONCLUSION:

The genetic expression of KDR is upregulated by shear stress and this response is time dependent.

PMID:
12626205
DOI:
10.1080/140174302762659102
[Indexed for MEDLINE]

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