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Methods Mol Biol. 2003;218:277-84.

Coimmunoprecipitation assay for the detection of kinase-substrate interactions.

Author information

1
Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.

Abstract

Coimmunoprecipitation is a powerful tool to study protein-protein interactions and can be used to test for the physical association between a known protein kinase and its substrate. In this chapter, the author describes a protocol for the preparation of a cell lysate, the immunoprecipitation of the antigen, and the analysis of the immune complex by gel electrophoresis and immunoblotting with antibodies that recognize the putative associated protein.

PMID:
12616727
[Indexed for MEDLINE]

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