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Vaccine. 2003 Mar 28;21(13-14):1363-70.

Super-activated interferon-regulatory factors can enhance plasmid immunization.

Author information

1
Department of Pathology and Molecular Medicine, Center for Gene Therapeutics, McMaster University, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5. bramsonj@mcmaster.ca

Abstract

We have been investigating the adjuvant properties of two super-activated interferon-regulatory factors (IRFs), IRF-3(5D) and IRF7/3A, identified in our previous studies of structure-function relationships, for enhancing plasmid vaccines. Intramuscular injection of plasmid cocktails encoding IRF-3(5D) and IRF7/3A molecules elicited cytotoxic T cell responses in over 80% of mice following a single immunization compared to a 20% response-rate using a control cocktail. Most interestingly, greater than 60% of mice immunized with the super-activated IRFs developed antigen-specific antibodies compared to 0% of the mice in the control group. Finally, vaccines which incorporated the super-activated IRFs provided greater protection against challenge with a recombinant vaccinia virus. These results support further investigation of the potential of these agents as adjuvants for genetic immunization.

PMID:
12615431
[Indexed for MEDLINE]

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