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Vaccine. 2003 Mar 28;21(13-14):1327-35.

Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice.

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Laboratory of Autoimmunity and Immunoregulation, Department of Immunology, Oswaldo Cruz Institute, Fiocruz. Av Brasil, 4365, Rio de Janeiro 21045-900, Brazil.


BALB/c mice were intramuscularly immunized with low doses (25-50microg) of DNA cocktail containing plasmids encoding the full-length SAG1/P30 and the 196-561 terminal sequence of ROP2 genes. This immunization resulted in a Th1-type response with predominance of IgG2a and a specific T-cell proliferation with high levels of interferon-gamma (IFN-gamma) secretion, whereas no IL-4 was detected. Moreover, DNA cocktail immunization induced a long-lasting protection against a lethal challenge with the highly virulent Toxoplasma gondii RH strain, whereas low doses of single genes were not protective. These results support further investigations to achieve a multigene anti-T. gondii DNA vaccine.

[Indexed for MEDLINE]

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