Format

Send to

Choose Destination
EMBO Rep. 2003 Feb;4(2):205-9.

A single gene that promotes interaction of a phytopathogenic bacterium with its insect vector, Drosophila melanogaster.

Author information

1
Centre de Génétique Moléculaire du CNRS, Avenue de la Terrasse, Bât. 26, F-91198 Gif-sur-Yvette Cedex, France.

Abstract

Insects are major vectors of plant and animal disease, and bacterial phytopathogens are often disseminated by flies. We have previously reported that some isolates of the phytopathogenic bacterial species Erwinia carotovora infect Drosophila and activate an immune response. Using a genetic screen, we have now identified two genes that are required by E. carotovora to infect Drosophila. One of these genes has a regulatory role whereas the other, evf, confers an infectious phenotype: its transfer to non-infectious Erwinia strains or to several enterobacteria improves survival in the gut and triggers the immune response. Overexpression of Erwinia virulence factor (evf) allowed bacteria to colonize the apical side of the gut epithelium and in some cases to spread to the body cavity. Our results demonstrate a specific interaction between plant pathogens and flies that promote their dissemination.

PMID:
12612613
PMCID:
PMC1315828
DOI:
10.1038/sj.embor.embor730
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center